Semen quality, Preservation and Artificial Insemination
Components of semen & Composition of spermatozoa
Chemical and physical properties of ejaculated semen
Factors affecting semen in vivo and in vitro.
Factors affecting semen production and quality
Semen preservation
Composition of diluents
Sperm concentration, Transport of diluted semen
Deep freezing techniques in cows, sheep, goats, swine and poultry.
Detection of oestrus and time of insemination for better conception.
Previous Years Questions
2019
Define true anoestrus in cow with characteristics of ovary and narrate the strategic approach to manage it.
Discuss the significance of semen quality and enlist the associated tests for its evaluation. Write down the ‘cervical mucus penetration test’ in detail.
Enlist the properties of ideal dilutor used for deep-freezing of bull semen with composition of TRIS dilutor used for same purpose.
Calculate the dilution factor and volume of dilutor from the given information :
Ejaculate volume- 5 ml
Progressive sperm motility-80%
Sperm concentration- 1000 x 106 /ml
Use of mini French straw for semen freezing
2018
a) Define AI and mention the advantages and disadvantages of AI.
b) Write the causes and management of repeat breeding cases in cows.
c) Write the physicochemical properties of ejaculated semen in domestic animals.
d) Discuss different methods of oestrus detection in animals.
2017
a) Describe the semen collection procedure in sheep and cattle.
2016
Why is the precise detection of oestrus very crucial in animal reproduction? What are the various methods of oestrus detection?
Discuss the diluents used in cattle semen preservation. What are the precautions that are necessary during semen preservation and thawing?
2015
Name three diluters along with their properties and composition for cryopreservation of cattle bull semen.
What do you understand by spermatogenesis and spermiogenesis? Discuss the factors affecting the semen quality in buffaloes.
2014
Discuss the role of biotechnology in genetic improvement of diary animals. State the steps involved in embryo transfer technology and its application.
What is repeat breeding syndrome in bovines? Enlist the causes of repeat breeding.
2013
Enlist various causes of anoestrus in bovines and discuss its remedial measures.
Enlist the characteristics of a semen diluter. Calculate the dilution rate of semen with following information available:
Semen to be frozen in French mini straws.
Semen volume : 5 ml
Sperm concentration in neat semen: 1000 million/ml